Deciphering the requirements of Notch ligand Jagged1 for the subpopulations of liver perivascular mesenchyme in development and disease, PhD

Faculty of Science, Charles University, Czech Republic (the)

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The award	How you will study	Study duration	Course start	Domestic course fees	International course fees
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About Deciphering the requirements of Notch ligand Jagged1 for the subpopulations of liver perivascular mesenchyme in development and disease, PhD - at Faculty of Science, Charles University

Lab Background

Our lab aims to solve medically relevant basic biological questions, focusing on Notch signaling and Wnt/Notch crosstalk in development and disease.

We strive to achieve a better mechanistic insight into the processes co-regulated by direct protein-protein interaction between components of Notch and Wnt pathways and Notch ligand signaling. To achieve this, we use CRISPR/Cas9-based technologies, together with bulk, scRNA seq, and lineage tracing in mouse and human cell lines, as well as manipulation of relevant genes in the mouse model.

The proposed project aims to define and functionally test the intrinsic requirements of Jagged1 signaling within the subpopulations of liver perivascular mesenchyme in development and disease.

Projects take advantage of the known biliary atresia, and Allagile syndrome-causing mutations in Jagged1[1], which we recapitulated in vivo in mice. The inductive role of Jagged1 (expressed by the liver periportal mesenchymal cells) for bile duct development is well established[2], however, the nature and extent of its requirement within the mesenchymal populations is unknown. To dissect the role of Jagged1-Notch signaling in the mesenchyme, this project aims to use human disease-relevant mouse models in combination with lineage tracing and transcriptomics.

The position is a stipend + 50% contract covered by the PRIMUS/UNCE funding. Applicants will be encouraged and supported in seeking additional fellowship funding and collaborative projects abroad.

Techniques: confocal microscopy, lineage tracing, mouse in vivo experiments, cloning, WB, RNA scope, sc-RNA-seq, bulk RNAseq.

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Students may obtain the following scholarships:

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scholarship for outstanding academic achievement (“mark-based”);
bursary in a case worthy of special consideration;
bursary to support study abroad;
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bursary to support study in the Czech Republic;
bursary for excellent research, development, innovation, artistic, or other creative achievements contributing to enhanced knowledge;
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motivation bursary for 1st year bachelor’s programme students;
motivation bursary for 1st year post-bachelor’s programme students;
sports bursary;
doctoral bursary.

Certain scholarships are granted to students automatically (without student applications), whereas others are granted further to an application (electronic or paper, depending on the type of scholarship). Bursaries are neither taxed nor included in stated income. You can find answers to frequently asked questions regarding scholarships on the Faculty website (natur.cuni.cz/fakulta/studium/helpdesk/faq-stipendia; Czech only).

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